In Silico Dissection of Regulatory Regions of PHT Genes from Saccharum spp. Hybrid and Sorghum bicolor and Expression Analysis of PHT Promoters under Osmotic Stress Conditions in Tobacco

Phosphorus (P) is the second-most essential macronutrient required for growth and development of plants. It involved in a number cellular processes that contribute to plant’s development. This study investigated Saccharum spp. hybrid Sorghum bicolor promoter regions Phosphate transporters (PHT), viz., PHT1, PHT2, PHT3, PHT4, PHO1, through silico analysis. The transcription start sites (TSS), conserved motifs, CpG islands were studied using various computational techniques. distribution TSSs indicated highest prediction scores (1.0). MSh2 MSb4 recognized as common motifs PHT promoters, found with 85 100% percentage distribution. analysis revealed most genes had low density, indicating possible tissue-specific expression. promoters presence biotic- abiotic-stress-associated factor binding (TFbs) major factors (TFs), namely, AP2/ERF, bHLH, bZIP, MYB, NAC, WRKY. Therefore, in-silico helps us understand regulation mechanism phosphate transporter gene expression under stress management. 5′ regulatory region EaPHT was isolated from Erianthus, wild relative genus Saccharum. construct prepared transformed tobacco wherein drove GUS. Analysis GUS transgenic enhanced salt-stress conditions. first report isolation characterization Erianthus expected be useful crop